Tools: NeuronStudio (Beta)

Version 0.9.92, last updated on November 19, 2009.

System Requirements:
  • Windows 2000/XP/Vista (32-bit and 64-bit versions)
  • OpenGL capable video card (required for 3D Viewer only)
Online help manual.
View change log.

Welcome to NeuronStudio. This program is designed to allow reconstruction of neuronal structures from confocal and multi-photon images. It is a self-contained software package that is free, easy to use, and available for 64-bit computers. NeuronStudio provides tools for manual, semi-manual, and automatic tracing of the dendritic arbor as well as manual and automatic detection and classification of dendritic spines. In addition, advanced 2D and 3D visualization techniques facilitate the verification of the reconstruction, as well as allowing accurate manual editing. The output of the program is compatible with standard compartment modeling and morphometric software applications.

32-bit and 64-bit Support

For 32-bit operating systems use ns.exe. For 64-bit operating systems use ns64.exe (also bundled in zip file).


If you encounter a recurring error and/or bug, please contact the following...


We request that you cite the following papers when using the results of NeuronStudio in your own published work.

  • For reconstruction and analysis of dendritic spines:
    Rodriguez A., Ehlenberger D.B., Dickstein D.L., Hof P.R., Wearne S.L. Automated Three-Dimensional Detection and Shape Classification of Dendritic Spines from Fluorescence Microscopy Images. PLoS ONE 3(4): e1997 doi:10.1371/journal.pone.0001997 (2008).
  • For reconstruction and analysis of dendritic arbors:
    Wearne, S.L., Rodriguez, A., Ehlenberger, D.B., Rocher, A.B., Hendersion, S.C., and Hof, P.R. New Techniques for imaging, digitization and analysis of three-dimensional neural morphology on multiple scales. Neuroscience, 136, 661-680, (2005).

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* Please review the license terms before using this program.