Rendl Lab
Rendl Lab
  Hair Follicle Morphogenesis: Stem Cell Activation and Cell Fate Specification by Dermal Papilla Cells.  
Cell communication between tissue stem cells and their cellular microenvironment within so-called stem cell niches is critical for stem cell self-renewal, differentiation and thus overall tissue homeostasis. But how these specialized niche cells acquire their inductive properties generally remains unknown. The focus of the lab is to understand the general molecular mechanisms of niche cell fate specification that is required for stem cell activation and tissue formation. We use hair follicle development as an attractive model system where a stem cell niche is formed when specialized mesenchymal cells, called dermal papilla (DP) cells, send cues to multipotent long-lived epithelial stem cells, thereby regulating their proliferation and progenitor cell fate, both during embryonic follicle morphogenesis and during adult follicle regeneration (Fig. 1).
Figure 1. Cell communication (black arrows) between epithelial stem cells (SC), progeny and dermal papilla (DP) cells occurs throughout hair follicle development and regeneration.

We have recently developed transgenic mice that enabled us to isolate pure DP cells and to characterize their molecular signature.  Cell fate manipulation either by cytokine activation in vitro or by gene ablation in a novel in vitro/in vivo hybrid knockout assay then allowed us for the first time to functionally assess their stem cell-activating, hair inducing properties (Fig. 2). 

Figure 2. DP cell-specific ablation of Bmpr1a decreases hair formation. Bmpr1a knockout and control DP cells were grafted with keratinocytes onto the backs of Nude mice. Left: Side view of graft area after 3 weeks (macro). Right: Hematoxylin/eosin (H+E) staining of graft samples. Arrowheads point to few aberrant downgrowths.




The goal now is to systematically employ these tools to identify the specific molecular events that specify DP character that is crucial for regulating epithelial stem cells.  Specifically we focus on transcription factors from the DP signature.  We test whether they play a role in DP niche fate specification in reprogramming experiments in vitro and in gene knockout experiments in vivo with novel DP-specific Cre mice.





These experiments will identify key mechanisms in the formation of the hair follicle stem cell niche.  Such insights will have also the potential to advance our understanding of other developmental systems where mesenchymal-epithelial communication takes place within a stem cell microenvironment. In addition, these studies could lead to therapeutic strategies to regenerate hair and other tissues.

Last Updated: 04/29/2016

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Rendl Lab